Western Blot: Alkaline Phosphatase, Tissue Non-Specific RNAi [H00000249-R01V] - analysis of ALPL over-expressed 293 cell line. Lane 2 cotransfected with ALPL Validated Chimera RNAi Lane 1 non-transfected control. Blot probed with H00000249-M01. GAPDH ( 36.1 kDa ) used as loading control.